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2019-09-26 LI-COR 與 JBC期刊合作開發 - Western Blot 第一口碑線上學堂

JBC 表示建議使用LI-COR Odyssey 等具有寬廣動態範圍的成像平臺

How should blots be presented?
Shang Y et al J Biol Chem 2018 Feb 16;293(7):2395-07
• There should be sufficient area around the bands of interest; ideally, at least one MW marker above and below
the band(s) of interest should be noted.
• Immunoblots should include the positions of the MW markers. If any 2D gels are used, indicate the pI ranges.
• Images should be appropriately adjusted such that it retains sufficient background information.
Loading controls usually not linear in
range loaded on blots
Eaton SL et al PLoS One 2013 Aug 30;8(8):e72457
• Tubulin saturates at ~ 10 μg in this system, but this value can change depending on system and detection method
• To detect protein of interest, researchers likely to load more than 10 μg of total protein
• Total protein stain shows a broader linear range